National Cytometry Platform
The NCP provides the best technology coupled with a thoughtful experimental design and advanced data analysis approaches to the scientific community in Luxembourg.
activities
The core facility offers a wide array of services, and it is open to national and international scientific collaborations. The National Cytometry Platform (NCP) provides access to single-cell technologies based on flow cytometry, microscopy, image cytometry, and mass cytometry.
Technologies
Single-cell analysis
- Mass Cytometry
- CyTOF XT™ (135 channels for the simultaneous measurement of more than 40 cellular markers)
- Flow Cytometry (conventional fluorescence based analyzers)
- BD LSRFortessa (5 lasers: 355nm, 405nm, 488nm, 561nm, 628nm; 20 channels)
- 2 x Novocyte Quanteon (4 lasers: 405nm, 488nm, 561nm, 628nm; 27 channels)
- Single-cell sorting
- 2 x BD FACSymphony™ S6 Cell Sorter (5 lasers: 355nm, 405nm, 488nm, 561nm, 637nm; 24 channels)
- BD Influx (4 lasers: 355 nm, 405 nm, 488nm, 640 nm; 16 channels)
- Image Cytometry (combination of flow cytometry and microscopy for a detailed image of the cell and its intracellular components)
- Image Stream MarkII (5 lasers: 405nm, 488nm, 561nm, 640nm, 785nm; 12 fluorescence channels; 20x 40x 60x magnification)
- Single-cell partioning and barcoding
- Chromium iX
- Full Spectrum Flow Cytometry
- “ID7000™ Spectral Cell Analyzer (4 lasers: 405nm, 488nm, 561nm, 637nm; 112 channels)
Microscopy
- Confocal microscopy
- LSM880 FastAiry – Zeiss (5 lasers: 405nm, 488nm, 543nm, 594nm, 633nm)
- Visible and epifluorescence microscopy
- Axio Observer Z1 – Zeiss
- Evos – Thermofischer
- Eclipse NI-E- Nikon
Multiplex immune assay systems
- Multiplexing imager
- MESO QuickPlex SQ 120 – MSD (high multiplexing system based on electrochemiluminescence to perform assays in 10 x 96 or 4 x 384 format)
Data analysis strategy
To address the need to integrate multidimensional data, metadata, and clinical information, we propose a strategy based on Business Intelligence described in a recent publication (https://pubmed.ncbi.nlm.nih.gov/33957013/ and https://public.tableau.com/app/profile/fanny2212).
The facility also offers the possibility of analyzing data with known software (FlowJo, Cytobank, Infinicyt, and CellEngine) and assisting with statistical evaluation of data (Qluocore, GraphPad Prism).
Training
The National Cytometry Platform provides training in cytometry and multidimensional data analysis to the scientific community in Luxembourg and the international community towards collaborations with different Institutes and Universities.
Cosma
Projects & clinical trials
Featured team members
Scientific publications
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Adenoviral delivery of the CIITA transgene induces T-cell-mediated killing in glioblastoma organoids – 13/11/2024
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Is the tumor cell side of the immunological synapse a polarized secretory domain? – 24/09/2024
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To the editor – 05/06/2024
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A comprehensive guide to study the immunological synapse using imaging flow cytometry – 03/04/2024
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Multiomics approaches disclose very-early molecular and cellular switches during insect-venom allergen-specific immunotherapy – 22/02/2024
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Proteome analysis of propolis deciphering the origin and function of its proteins – 01/02/2024
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Early-to-mid stage idiopathic Parkinson’s disease shows enhanced cytotoxicity and differentiation in CD8 T-cells in females – 20/11/2023
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Actin cytoskeleton remodeling at the cancer cell side of the immunological synapse – 21/09/2023
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Correlative light and electron microscopy to explore the lytic immunological synapse between natural killer cells and cancer cells – 05/06/2023
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Actin cytoskeleton depolymerization increases matrix metalloproteinase gene expression in breast cancer cells by promoting translocation of cysteine-rich protein 2 to the nucleus – 15/05/2023