National Cytometry Platform

The NCP provides the best technology coupled with a thoughtful experimental design and advanced data analysis approaches to the scientific community in Luxembourg.

activities

The core facility offers a wide array of services, and it is open to national and international scientific collaborations. The National Cytometry Platform (NCP) provides access to single-cell technologies based on flow cytometry, microscopy, image cytometry, and mass cytometry. 

Technologies

Single-cell analysis

  • Mass Cytometry
    • Helios (135 channels for the simultaneous measurement of more than 40 cellular markers)
  • Flow Cytometry (conventional fluorescence based analyzers)
    • BD LSRFortessa (5 lasers: 355nm, 405nm, 488nm, 561nm, 628nm; 20 channels)
    • 2 x Novocyte Quanteon (4 lasers: 405nm, 488nm, 561nm, 628nm; 27 channels)
    • BD Canto (2 lasers : 488nm,  633nm; 8 channels)
  • Single-cell sorting
    • BD FACS Aria II (5 lasers: 355nm, 405nm, 488nm, 561nm, 628nm; 20 channels)
    • BD Influx (4 lasers: 355 nm, 405 nm, 488nm, 640 nm; 16 channels)
  • Image Cytometry (combination of flow cytometry and microscopy for a detailed image of the cell and its intracellular components)
    • Image Stream MarkII (5 lasers: 405nm, 488nm, 561nm, 640nm, 785nm; 12 fluorescence channels; 20x 40x 60x magnification)

Microscopy

  • Confocal microscopy
    • LSM880 FastAiry – Zeiss (5 lasers: 405nm, 488nm, 543nm, 594nm, 633nm)
  • Visible and epifluorescence microscopy
    • Axio Observer Z1 – Zeiss
    • Evos – Thermofischer
    • Eclipse NI-E- Nikon

Multiplex immune assay systems

  • Multiplexing imager
    • MESO QuickPlex SQ 120 – MSD (high multiplexing system based on electrochemiluminescence to perform assays in 10 x 96 or 4 x 384 format)

Data analysis strategy

To address the need to integrate multidimensional data, metadata, and clinical information, we propose a strategy based on Business Intelligence described in a recent publication (https://pubmed.ncbi.nlm.nih.gov/33957013/ and https://public.tableau.com/app/profile/fanny2212).

The facility also offers the possibility of analyzing data with known software (FlowJo, Cytobank, Infinicyt, and CellEngine) and assisting with statistical evaluation of data (Qluocore, GraphPad Prism).

Training

The National Cytometry Platform provides training in cytometry and multidimensional data analysis to the scientific community in Luxembourg and the international community towards collaborations with different Institutes and Universities.

Antonio
Cosma

Projects & clinical trials

Scientific publications

LIH researchers strive to generate meaningful and disease-relevant knowledge and results for patients. Their findings are regularly published in prestigious international peer-reviewed journals, thereby extending the reach and visibility of the institute’s impact.