Preclinical services for advancing cancer treatment for patients with brain tumours.
Oudin A, Baus V, Barthelemy V, Fabian C, Klein E, Dieterle M, Wantz M, Hau AC, Dording C, Bernard A, Michelucci A, Yabo YA, Kanli G, Keunen O, Bjerkvig R, Niclou SP, Golebiewska A. Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors. STAR Protoc. 2021 May 12;2(2):100534. doi: 10.1016/j.xpro.2021.100534
Detailed protocol for model derivation, MRI and ex-vivo functional studies
Yabo YA, Moreno-Sanchez PM, Pires-Afonso Y, Kaoma T, Nosirov B, Scafidi A, Ermini L, Lipsa A, Kyriakis D, Grzyb K, Poovathingal SK, Poli A, Muller A, Toth R, Oudin A, Klink B, Berchem G, Berthold C, Hertel F, Mittelbronn M, Heiland DH, Skupin A, Nazarov PV, Niclou SP, Michelucci A, Golebiewska A; Glioblastoma-instructed microglia transit to heterogeneous phenotypic states with phagocytic and dendritic cell-like features in patient tumors and patient-derived orthotopic xenografts; BioRxiv 2023. https://doi.org/10.1101/2023.03.05.531162
Most updated characterization of LIH-NORLUX glioma cohort. Detailed characterization of tumor microenvironment created in PDOX models in the mouse brain.
Golebiewska A, Hau A-C, Oudin A, Stieber D, Yabo Y A, Baus V, Barthelemy V, Klein E, Bougnaud S, Keunen O, Wantz M, Michelucci A, Neirinckx V, Muller A, Kaoma T, Nazarov P V, Azuaje F, de Falco A, Flies B, Poovathingal S, Arns T, Grzyb K, Mock A, Herold-Mende C, Steino A, Brown D, May P, Miletic H, Noushmehr H, Kwon Y-J, Jahn W, Klink B, Tanner G, Stead LF, Mittelbronn M, Skupin A, Hertel F, Bjerkvig R and Niclou SP; Patient-derived organoids and orthotopic xenografts of primary and recurrent gliomas represent relevant patient avatars for precision oncology. Acta Neuropathologica, Oct 2020. https://pubmed.ncbi.nlm.nih.gov/33009951/
In-depth characterization of our preclinical cohort at histopathological and multi-omics levels. The article presents examples of in vivo and ex vivo efficacy studies in the context of standard-of-care and personalized treatments
Oudin A, Moreno-Sanchez PM, Baus V, Niclou SP, Golebiewska A Magnetic resonance imaging-guided intracranial resection of glioblastoma tumors in patient-derived orthotopic xenografts leads to clinically relevant tumor recurrence, BMC Cancer. 2023 doi: 10.1186/s12885-023-11774-6
Our advanced protocol for MRI-guided neurosurgery in PDOXs
Klein E, Hau A-C, Oudin A, Golebiewska A, Niclou SP; Glioblastoma Organoids: Pre-Clinical Applications and Challenges in the Context of Immunotherapy. Frontiers in Oncology. 2020. https://pubmed.ncbi.nlm.nih.gov/33364198/
Our review describing glioblastoma organoids and PDOXs available for efficacy studies and immunotherapeutic interventions
Additional publications: Please visit the NORLUX web page to consult the publications.
