Preclinical services for advancing cancer treatment for patients with brain tumours.
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Golebiewska A, Hau A-C, Oudin A, Stieber D, Yabo Y A, Baus V, Barthelemy V, Klein E, Bougnaud S, Keunen O, Wantz M, Michelucci A, Neirinckx V, Muller A, Kaoma T, Nazarov P V, Azuaje F, de Falco A, Flies B, Poovathingal S, Arns T, Grzyb K, Mock A, Herold-Mende C, Steino A, Brown D, May P, Miletic H, Noushmehr H, Kwon Y-J, Jahn W, Klink B, Tanner G, Stead LF, Mittelbronn M, Skupin A, Hertel F, Bjerkvig R and Niclou SP; Patient-derived organoids and orthotopic xenografts of primary and recurrent gliomas represent relevant patient avatars for precision oncology. Acta Neuropathologica, Oct 2020. | Link
In-depth characterization of our preclinical cohort at histopathological and multi-omics levels. The article presents examples of in vivo and ex vivo efficacy studies in the context of standard-of-care and personalized treatments
Oudin A, Baus V, Barthelemy V, Fabian C, Klein E, Dieterle M, Wantz M, Hau AC, Dording C, Bernard A, Michelucci A, Yabo YA, Kanli G, Keunen O, Bjerkvig R, Niclou SP, Golebiewska A. Protocol for derivation of organoids and patient-derived orthotopic xenografts from glioma patient tumors. STAR Protoc. 2021 May 12;2(2):100534. | Link
Detailed protocol for model derivation, MRI and ex-vivo functional studies
Oudin A, Moreno-Sanchez PM, Baus V, Niclou SP, Golebiewska A. Magnetic resonance imaging-guided intracranial resection of glioblastoma tumors in patient-derived orthotopic xenografts leads to clinically relevant tumor recurrence, BMC Cancer. 2023 | Link
Our advanced protocol for MRI-guided neurosurgery in PDOXs
Yabo YA, Moreno-Sanchez PM, Pires-Afonso Y, Kaoma T, Nosirov B, Scafidi A, Ermini L, Lipsa A, Kyriakis D, Grzyb K, Poovathingal SK, Poli A, Muller A, Toth R, Oudin A, Klink B, Berchem G, Berthold C, Hertel F, Mittelbronn M, Heiland DH, Skupin A, Nazarov PV, Niclou SP, Michelucci A, Golebiewska A; Glioblastoma-instructed microglia transition to heterogeneous phenotypic states with phagocytic and dendritic cell-like features in patient tumors and patient-derived orthotopic xenografts; Genome Medicine 2024. | Link
Most updated characterization of LIH-NORLUX glioma cohort. Detailed characterization of tumor microenvironment created in PDOX models in the mouse brain.
Neises L, Delbrouck C, Schuster A, Rezaipour M, Eiden K, Oudin A, Fabian C, Niclou SP, Golebiewska A*, Meiser J*.; Protocol using ex vivo mouse brain slice culture mimicking in vivo conditions to study tumor growth and cell motility of glioblastoma cells. STAR Protoc. 2024 Oct 18;5(4):103401. | Link
Our protocol for brain slice cultures ex vivo
Zhou W, Martinez-Garcia E, Sarnow K, Kanli G, Nazarov PV, Li Y, Schwab S, Meiser J, Jaeger C, Mieczkowski J, Misztak A, Thorsen FA, Grützmann K, Mihaljevic B, van Loon B, Hossain JA, Zhang Y, Xue Z, Li W, Moreino SS, Golebiewska A, Niclou SP, Bjørås M, Tardito S, Joesph JV, Lunavat TR, Saed HS, Bahador M, Han M, Fabian C, Miletic H, Li X, Dittmar G, Keunen O, Klink B, Wang J, Bjerkvig R. Development of a highly differentiated rat brain organoid model for exploring glioblastoma invasion dynamics and therapy. Neuro Oncol. 2025 Nov 24:noaf271. PMID: 41284925. | Link
Our advanced protocol for co-cultures with healthy brain organoids
Ermini, L., A. Lipsa, A.-C. Hau, I. Krokhmal, B. Nosirov, R. Toth, E. Klein, A. Oudin, L. Houben, S. Fritah, C. Herold-Mende, K. B. M. Frauenknecht, M. Mittelbronn, G. Berchem, F. Hertel, F. Malta T. M., Nazarov P. V., Niclou S. P. and Golebiewska A. (2025). „Integrative multi-omics combined with functional pharmacological profiling in patient-derived organoids identifies personalized therapeutic vulnerabilities of adult high-grade gliomas.“ bioRxiv: 2025.2009.2009.675145.
Our high-throughput drug screening protocol
Moreno-Sanchez PM, Oudin A, Kisakol B, Dussmann H, Klein E, Baus V, Rolin C, Seguin-Devaux C, Rezaeipour M, Poli A, Michelucci A, Paggetti J, Moussay E, Prehn JHM , Niclou SP, Golebiewska A; Humanized glioblastoma patient-derived orthotopic xenografts recreate a locally immunosuppressed human immune ecosystem amenable to immunotherapeutic modulation; bioRxiv 2025.11.20.689484. | Link
Our advanced in vivo modelling in humanized mice
Rezaeipour, M., de Lucas Sanz, M., Allahverdi, H., Triballier, G., Klein, E., Salvato, I., Moreno-Sanchez, P. M., Nosirov, B., Nazarov, P. V., Kisakol, B., Dussmann, H., Connolly, C., Byrne, A. T., Prehn, J. H. M., Brenner, D., Janji, B., Niclou, S. P. & Golebiewska, A. Advanced 3D patient-derived glioblastoma organoid-immune cell co-cultures reveal preserved MHC-I tumor cell surface expression and tumor cell-driven T-cell dysfunction. Journal of Neuroinflammation (2026). | Link
Our advanced tumor-T-cell co-culture protocol
Our review describing glioblastoma organoids and PDOXs available for efficacy studies and immunotherapeutic interventions:
De Lucas Sanz M., Niclou S.P, Golebiewska A., Modeling brain tumors with organoids: toward precision medicine in neuro-oncology. Nature Reviews Neurology, 2026. | Link
Lipsa A., Triballier G, Golebiewska A; Exploring glioblastoma microenvironment using organoids: opportunities, limitations, and emerging concepts, Seminars in Cancer Biology, Volumes 123–124, 2026, Pages 44-60, ISSN 1044-579X. | Link
Connor K, Golebiewska A, Byrne AT. Challenging the status quo to improve the translational potential of preclinical oncology studies; Nature Reviews Cancer. 2024 Oct 7. | Link
Klein E, Hau A-C, Oudin A, Golebiewska A, Niclou SP; Glioblastoma Organoids: Pre-Clinical Applications and Challenges in the Context of Immunotherapy. Frontiers in Oncology. 2020. | Link
Additional publications: Please visit the NORLUX web page to consult the publications.
