The platform is composed of:
A confocal microscope LSM880 FastAiry from Carl Zeiss. It is a versatile system with high sensitivity and resolution that allows imaging in 2D and 3D of fixed and living biological samples. The Airy scan module detector represents a major technical innovation allowing both high-resolution imaging (as compared to a classical confocal microscope, it increases the resolution by a factor of 1.7, i.e. from 240 nm to 140 nm) and high sensitivity detection.
The new Zeiss LSM880 Fast Airy microscope was co-financed by a FEDER project (Fonds Européen de Développement Régional, programme : Recherche et Innovation « Investissement pour la croissance et l’Emploi » 2014-2020 / Projet N°: «2016-01-12»). The financing was obtained to replace the former confocal microscope of the LIH platform beginning of 2017 to provide state-of-the-art technologies and high-end solutions to researchers from LIH.
An epi-fluorescence microscope from Zeiss equipped with a LSM510 META confocal module and a TIRF module for evanescent wave microscopy.
An Incucyte Zoom automated system for live cell imaging from Satorius, which allows long term imaging over several days to follow proliferation, spheroid growth, cell migration and invasion with a scratch wound and chemotaxis module.
An analysis station equipped with specific software licenses such as Huygens (Svi, for image deconvolution) and Imaris (Bitplane for 3D analysis and reconstruction).
- Technical support for microscopes handling and cell imaging in 2D and 3D (confocal, TIRF, epi-fluorescence and brightfield microscopy).
- Immuno-fluorescence imaging and advanced fluorescence technics (FRAP, FRET, photo-activation, spectral unmixing, ratiometric dye measurement).
- Live cell imaging by confocal microscopy (Temperature, CO2 and O2 control) or by standard fluorescence with the Incucyte system (long term imaging).
- Training and assistance using image treatment software for image handling, data quantification, cell tracking, image deconvolution and 3D reconstruction.
- Training in confocal microscopy and equipment operation for students and new users.
Head of Support Service Unit
Dr Hoffmann Céline has been in charge of the confocal microscopy platform since 2005 within the department of Oncology. Her research is focused on cytoskeleton dynamics and remodelling in cancer cells, with a special emphasis on cell migration/invasion processes.