Lecture - Prof Dr Klaus Pfeffer - Interferon inducible guanylate binding proteins (GBPS) in immune defense
15/09/2016 13:00 to 15/09/2016 16:30 (Europe/Luxembourg)
Host defense against invading pathogens is critically dependent upon interferon induced anti-microbial effector programs. Recently, we have discovered that vast number of interferon induced genes are GTPases belonging to the family of guanylate binding proteins (GBPs). GBPs are essential for immunity against intracellular pathogens, especially for Toxoplasma gondii control. Now the molecular interactions of murine GBPs (mGBP1/2/3/5/6), homo- and hetero-multimerization properties of mGBP2 and its function in parasite killing were investigated by mutational, Multiparameter Fluorescence Image Spectroscopy, and live cell microscopy methodologies. Control of T. gondii replication by mGBP2 requires GTP hydrolysis and isoprenylation thus, enabling reversible oligomerization in vesicle-like structures. mGBP2 undergoes structural transitions between monomeric, dimeric and oligomeric states visualized by quantitative FRET analysis. mGBPs reside in at least two discrete subcellular reservoirs and attack the parasitophorous vacuole membrane (PVM) as orchestrated, supramolecular complexes forming large, densely packed multimers comprising up to several thousand monomers. This dramatic mGBP enrichment results in the loss of PVM integrity, followed by a direct assault of mGBP2 upon the plasma membrane of the parasite. These discoveries provide vital dynamic and molecular perceptions into cell-autonomous immunity.
13:00 - 14:30 : LECTURE
Lycée Technique d'Esch/Alzette - Salle de projection audiovisuelle
15:00 - 16:30 : 'meet & greet'
House of BioHealth
Room Françoise Barré-Sinoussi
Registration required for the 'Meet & Greet' - send us an email.
About the Lecture & Workshop series - Infection & Immunity
The LIH Lecture Series and Workshops in Infection and Immunity, supported by the FNR, are gathering internationally recognised speakers to address topics around Infection and Immunity. Twelve lectures will be organised in 2016, followed by workshops especially dedicated to early-stage researchers.
Attendance to the lectures and workshops are free of charge. Should you be interested in registering for the workshops, please feel free to do so by sending us an email.
Download the whole programme for 2016 here!