The emerging landscape of single-molecule protein sequencing technologies.

  • Quantitative Biology Unit
June 01, 2021 By:
  • Alfaro JA
  • Bohlander P
  • Dai M
  • Filius M
  • Howard CJ
  • van Kooten XF
  • Ohayon S
  • Pomorski A
  • Schmid S
  • Aksimentiev A
  • Anslyn EV
  • Bedran G
  • Cao C
  • Chinappi M
  • Coyaud E
  • Dekker C
  • Dittmar G
  • Drachman N
  • Eelkema R
  • Goodlett D
  • Hentz S
  • Kalathiya U
  • Kelleher NL
  • Kelly RT
  • Kelman Z
  • Kim SH
  • Kuster B
  • Rodriguez-Larrea D
  • Lindsay S
  • Maglia G
  • Marcotte EM
  • Marino JP
  • Masselon C
  • Mayer M
  • Samaras P
  • Sarthak K
  • Sepiashvili L
  • Stein D
  • Wanunu M
  • Wilhelm M
  • Yin P
  • Meller A
  • Joo C.

Single-cell profiling methods have had a profound impact on the understanding of cellular heterogeneity. While genomes and transcriptomes can be explored at the single-cell level, single-cell profiling of proteomes is not yet established. Here we describe new single-molecule protein sequencing and identification technologies alongside innovations in mass spectrometry that will eventually enable broad sequence coverage in single-cell profiling. These technologies will in turn facilitate biological discovery and open new avenues for ultrasensitive disease diagnostics.

2021 Jun. Nat Methods.18(6):604-617. Epub 2021 Jun 7.
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