[Molecular epidemiology of parvovirus infection in Belarus].
- Clinical and Applied Virology
The paper analyzes a 994 nucleotide fragment of the NS1/VP1u region junction of 84 parvovirus B19 samples obtained from the sera of erythema infectiosum patients in Belarus in 2006. All the strains belong to genotype 1 as defined by Servant et al. (2002) and form two major clusters within this genotype (1A and 1B) with a clearly distinct geographic distribution. Cluster 1A mainly included B19 strains from Minsk where an outbreak of erythema infectiosum was observed during sample collection. Cluster 1B comprises parvovirus B19 strains obtained from sporadic cases in different parts of the country. The nucleotide variability within cluster 1B (1.1%) was almost two times higher than that within cluster 1A (0.6%). The comparison of the Belarus strains with all parvovirus B19 sequences from the GenBank revealed 22 unique nucleotide substitutions in the new strains, 18 (81.8%) of which were nonsynonymous. A high percentage of parvovirus B19 IgM positive sera were also PCR positive (94.0%; n = 63/67) indicating that both methods are suitable for diagnosis of the infection.